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中国人民解放军总医院老年心血管病研究所
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中华老年多器官疾病杂志编辑委员会
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创刊人 王士雯
总编辑 范利
副总编辑 陈韵岱
执行主编 叶大训
编辑部主任 王雪萍
ISSN 1671-5403
CN 11-4786
创刊时间 2002年
出版周期 月刊
邮发代号 82-408
友情链接
杨伟伟,李艳飞,钱素敏.黄芪甲苷对甲状腺功能减退雌性大鼠子宫氧化应激反应的影响[J].中华老年多器官疾病杂志,2021,20(4):295~299
黄芪甲苷对甲状腺功能减退雌性大鼠子宫氧化应激反应的影响
Effects of astragaloside Ⅳ on oxidative stress of uterus in female rats with hypothyroidism
投稿时间:2020-09-06  
DOI:10.11915/j.issn.1671-5403.2021.04.061
中文关键词:  子宫;黄芪甲苷;甲状腺功能减退;氧化应激反应;能量代谢
英文关键词:uterus; astragaloside Ⅳ; hypothyroidism; oxidative stress reaction; energy metabolism Corresponding author:QIAN Su-Min,E-mail:qsmlm@126.com〖FL
基金项目:
作者单位E-mail
杨伟伟 沧州市中心医院妇二科,河北 沧州 061001 qsmlm@126.comeffects 
李艳飞 沧州市中心医院妇二科,河北 沧州 061001 qsmlm@126.comeffects 
钱素敏 沧州市中心医院妇二科,河北 沧州 061001 qsmlm@126.comeffects 
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中文摘要:
      目的 观察黄芪甲苷(AST)对甲状腺功能减退雌性大鼠子宫氧化应激反应的影响。方法 将60只大鼠[4周龄,体质量(110±10) g],采用随机数表法分为对照组,模型组,AST 20、40、80mg/kg组和左甲状腺素钠9×10-3mg/kg组,给予0.1%丙基硫氧嘧啶复制甲状腺功能减退动物模型,以左甲状腺素钠作为阳性对照药,检测各组大鼠血清髓过氧化物酶(MPO)、过氧化氢酶(CAT)活性和一氧化氮(NO)含量;子宫组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,丙二醛(MDA)、乳酸脱氢酶(LDH)含量,钙离子(Ca2+)浓度和高能磷酸化合物(HEP)水平;离体子宫平滑肌收缩张力(CT)、收缩频率(CF)和子宫活动力(UM)。采用SPSS 17.0统计软件进行数据分析。多组间比较采用方差分析,组间两两比较采用SNK检验。结果 AST组大鼠血清MPO活性和NO含量降低,CAT活性升高(P<0.05);子宫组织中三磷酸腺苷、二磷酸腺苷、一磷酸腺苷、总腺嘌呤核苷酸和SOD、GSH-Px明显升高,LDH和MDA明显降低(P<0.05);离体子宫平滑肌CT、CF、UM以及Ca2+浓度均明显升高(P<0.05),以AST 80mg/kg效果最为显著。结论 AST具有抑制甲状腺功能减退雌性大鼠子宫氧化应激损伤的作用,可能与其能够有效改善抗氧化酶活性、开放Ca2+通道、增强离体子宫平滑肌的敏感性、并促进能量代谢有关。
英文摘要:
      Objective To observe the effect of astragaloside IV (AST) on oxidative stress of uterus in female rats with hypothyroidism. Methods A total of 60 female rats [4 weeks old, weighing (110±10) g] were randomly divided into control group, model group, AST treatment groups (20,40 and 80 mg/kg) and positive drug group (levothyroxine sodium, 9×10-3 mg/kg). The rat model of thyroid dysfunction was induced with intragastric infusion of 0.1% propylthiouracil at a dose of 10 ml/kg. Then the serum contents of related indicators were detected with corresponding test kits, including myeloperoxidase (MPO), catalase (CAT) and nitric oxide (NO). The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), the contents of malondialdehyde (MDA), lactate dehydrogenase (LDH) and Ca2+ in the uterine tissues were measured with corresponding test kits, and the level of high energy phosphates (HEP) was detected with high performance liquid chromatography. The contraction tension (CT) and contraction frequency (CF) of the smooth muscle samples derived from isolated uterine were measured, and uterine motility (UM) was calculated. Statistical analysis were performed by SPSS statistics 17.0. ANOVA was used for comparison among groups, and adjusted SNK test was used for comparison between two groups. Results MPO activity and NO level were decreased significantly, and CAT activity was enhanced obviously in AST treatment groups than the model group (P<0.05). The activities of 3′-adenylate triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), total adenine nucleotides (TAN), SOD and GSH-Px were also increased, and the contents of LDH and MDA were reduced significantly (P<0.05). AST treatment also resulted in significant elevations in CT, CF and UM of the isolated uterine smooth muscle and the concentration of Ca2+when compared with the model group (P<0.05), especially when the dose of AST was 80 mg/kg. Conclusion AST inhibits the effects on oxidative stress injury of uterus in female rats induced by hypothyroidism, which may be related to its enhancing the activity of antioxidant enzymes, opening Ca2+ channel and promoting energy metabolism.
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