在线办公
期刊论坛
主 管
中国人民解放军总医院
主 办
中国人民解放军总医院老年心血管病研究所
中国科技出版传媒股份有限公司
编 辑
中华老年多器官疾病杂志编辑委员会
100853, 北京市复兴路28号
电话:010-66936756
传真:010-66936756
E-mail: zhlndqg@mode301.cn
创刊人 王士雯
总编辑 范利
副总编辑 陈韵岱
执行主编 叶大训
编辑部主任 王雪萍
ISSN 1671-5403
CN 11-4786
创刊时间 2002年
出版周期 月刊
邮发代号 82-408
友情链接
杨婉琳,杨德辉,汤瑜昳,易定锋.褪黑素干预动脉性肺动脉高压大鼠的实验研究[J].中华老年多器官疾病杂志,2019,18(10):773~777
褪黑素干预动脉性肺动脉高压大鼠的实验研究
Interventional effect of melatonin in rat model of pulmonary arterial hypertension
投稿时间:2019-03-09  
DOI:10.11915/j.issn.1671-5403.2019.10.167
中文关键词:  褪黑素;野百合碱;动脉性肺动脉高压;环氧合酶-2
英文关键词:melatonin; monocrotaline; pulmonary arterial hypertension; cyclooxygenase-2 This work was supported by the Type C Project of Hunan Provincial Health Commission
基金项目:湖南省卫健委C类项目(C2019087)
作者单位E-mail
杨婉琳 解放军联勤保障部队第九二一医院心血管内科,长沙 410022  
杨德辉 解放军联勤保障部队第九二一医院心血管内科,长沙 410022 980450828@qq.com 
汤瑜昳 解放军联勤保障部队第九二一医院心血管内科,长沙 410022  
易定锋 解放军联勤保障部队第九二一医院心血管内科,长沙 410022  
摘要点击次数: 45
全文下载次数: 53
中文摘要:
      目的 探讨褪黑素(MEL)干预对野百合碱(MCT)诱导的动脉性肺动脉高压(PAH)大鼠肺动脉压及肺组织环氧合酶-2(COX-2)表达情况的影响。方法 36只大鼠按随机数表法分为对照组、造模组(MCT)、干预组(MCT+MEL)。造模组和干预组分别按2.5ml/kg(50mg/kg,溶于无水乙醇+生理盐水混合液)规格腹膜内注射MCT溶液。对照组按2.5ml/kg接受腹膜内注射溶媒。造模后第1~28天,干预组予以MEL 10ml/kg (10mg/kg,溶于无水乙醇+生理盐水混合液)腹膜内注射,1次/d,对照组和造模组予以单位等体积溶媒腹膜内注射,1次/d。测量并记录3组大鼠平均肺动脉压(mPAP)、右心室肥厚指数(RVHI)、肺小动脉管壁厚度占血管外径的百分比(WT%)、肺小动脉血管壁面积占血管总面积的百分比(WA%)及肺小动脉COX-2平均光密度值(AOD)。应用SPSS 22.0统计软件对数据进行分析。结果 与对照组比较,造模组大鼠mPAP、RVHI、WT%、WA%、AOD显著升高;与造模组比较,干预组大鼠上述指标均有不同程度降低,但仍高于对照组,差异均具有统计学意义(P<0.001)。相关性分析显示,AOD与mPAP、RVHI、WT%、WA%呈显著正相关(r依次为0.836、0.749、0.823、0.821,P均<0.01)。结论 MEL能够降低MCT诱导的PAH大鼠的肺动脉压,改善肺血管重塑,这可能与其抑制COX-2表达水平有关。
英文摘要:
      Objective To investigate the effects of melatonin(MEL) on pulmonary artery pressure and expression of cyclooxygenase-2 (COX-2) in rats with monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH). Methods Thirty-six rats were randomly divided into control group, model group (MCT), and intervention group (MCT+MEL). MCT of 2.5ml/kg (50mg/kg, dissolved in mixture of anhydrous ethanol and normal saline) was injected intraperitoneally into the rats of the model group and intervention group to establish PAH. The rats from the control group received intraperitoneal injection of 2.5ml/kg of the solvent. From the 1st day to the 28th day after the injection, MEL of 10ml/kg (10mg/kg, dissolved in mixture of anhydrous ethanol and normal saline) was injected intraperitoneally in the intervention group, once a day, and the control group and the model group were given same dose of the solvent. Mean pulmonary artery pressure (mPAP), right ventricular hypertrophy index (RVHI), vascular wall thickness/vascular external diameter (WT%), vascular wall area/total vascular area(WA%), and the average optical density(AOD) of COX-2 expression were measured and recorded. SPSS statistics 22.0 was used for data analysis. Results Compared with control group, the mPAP, RVHI, WT%, WA% and AOD were significantly higher in the model group, but the above indices were reduced in the intervention group when compared with those of the model group, but still higher than those in control group with statistical significance (P<0.001). Correlation analysis showed that AOD was positively correlated with mPAP, RVHIV, WT% and WA% (r=0.836,0.749,0.823,0.821 respectively, P<0.01). Conclusion MEL can reduce MCT-induced PAH and improve pulmonary vascular remodeling in rats, which may be associated with its inhibiting COX-2 expression.
查看全文    下载PDF阅读器
关闭